Read e-book online A Novel Heme-Thiolate Peroxygenase AaeAPO and Its PDF

By Xiaoshi Wang

ISBN-10: 3319032356

ISBN-13: 9783319032351

during this thesis, Xiaoshi Wang investigates the functionality and mechanism of a newly came upon heme-thiolate peroxygenase, AaeAPO. This enzyme category comes from Agrocybe aegerita and is utilized in the conversion of inert hydrocarbons to alcohols. Xiaoshi's paintings specializes in an extracellular P450 enzyme which isn't constrained in its balance and shortage of solubility and hence is proper for common business use. the writer demonstrates that the peroxygenase catalyzes quite a lot of reactions. occasionally the writer even describes very tough ameliorations in molecules which are hugely inert. Her particular investigations supply a mechanistic framework for a way the peroxygenase catalyzes one of these huge variety of reactions. an incredible spotlight of this thesis is the id of key short-lived intermediates within the catalytic cycle of the peroxygenase, utilizing fast kinetic and spectroscopic equipment, in addition to the elucidation of the thermodynamic houses of those high-energy intermediates. This paintings provides new perception into a tremendous classification of enzymes.

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A Novel Heme-Thiolate Peroxygenase AaeAPO and Its Implications for C-H Activation Chemistry (Springer Theses) by Xiaoshi Wang

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